تفاعل #76008

معادلة التفاعل

lithium chloride

sodium borohydride

ethyl (2R)-6-{[(benzyloxy)-carbonyl]amino}-2-{[(tert-butylamino)carbothioyl]-amino}hexanoate

borohydride

→

benzyl (5R)-5-{[(tert-butylamino)carbothioyl]amino}-6-hydroxyhexylcarbamate

المردود 60.3%

المذيبات

ethanol

tetrahydrofuran

dichloromethane ethyl acetate

ظروف التفاعل

المعالجة

1. 1
   درجة الحرارةcooled to between 0° C. and 5° C
2. 2
   درجة الحرارةthe mixture is maintained at about 80° C. for 4 hours
3. 3
   ترشيحThe reaction medium is filtered
4. 4
   غسيلthe filter cake is washed with ethanol
5. 5
   تركيزThe filtrate is concentrated under reduced pressure (5 kPa) at a temperature in the region of 40° C.
6. 6
   أخرىthe residue obtained
7. 7
   workup.WAITleft under cold conditions at about 5° C
8. 8
   ترشيحThe crystalline product is spin-filtered
9. 9
   أخرىpurified by chromatography at atmospheric pressure on a column of silica gel (particle size 40-63μ; mass of silica: 600 g)
10. 10
    غسيلeluting with a dichloromethane/ethyl acetate mixture (85/15 by volume)
11. 11
    workup.ADDITIONThe fractions containing the expected product
12. 12
    أخرىare collected
13. 13
    تركيزconcentrated under reduced pressure (5 kPa) at a temperature in the region of 40° C

الإجراء التجريبي

1.78 g of lithium chloride and then 1.6 g of sodium borohydride are added, under an inert atmosphere, to a stirred solution of 12.7 g of ethyl (2R)-6-{[(benzyloxy)-carbonyl]amino}-2-{[(tert-butylamino)carbothioyl]-amino}hexanoate in 130 cm3 of ethanol and 65 cm3 of tetrahydrofuran, cooled to between 0° C. and 5° C. After stirring for 16 hours at a temperature in the region of 20° C., a further 0.4 g of borohydride is added and the mixture is maintained at about 80° C. for 4 hours. The reaction medium is filtered and the filter cake is washed with ethanol. The filtrate is concentrated under reduced pressure (5 kPa) at a temperature in the region of 40° C. and the residue obtained is taken up in a dichloromethane/ethyl acetate mixture (85/15 by volume) and left under cold conditions at about 5° C. The crystalline product is spin-filtered and purified by chromatography at atmospheric pressure on a column of silica gel (particle size 40-63μ; mass of silica: 600 g), eluting with a dichloromethane/ethyl acetate mixture (85/15 by volume). The fractions containing the expected product are collected. These fractions are combined and then concentrated under reduced pressure (5 kPa) at a temperature in the region of 40° C. 6.9 g of benzyl (5R)-5-{[(tert-butylamino)carbothioyl]amino}-6-hydroxyhexylcarbamate are obtained in the form of a colorless oil. (αD20=+24.0±0.8 at a concentration of 0.5% in methanol).

المصدر