تفاعل #747439

معادلة التفاعل

5,5,5-trifluoro-2-oxopentanoic acid

pyridoxal phosphate

→

(R)-5,5,5-trifluoro-2-aminopentanoic acid

المذيبات

potassium phosphate

ظروف التفاعل

المعالجة

1. 1
   أخرىReaction

الإجراء التجريبي

A solution containing 5,5,5-trifluoro-2-oxopentanoic acid (100 mg, 0.588 mmoles), R,S-alanine (200 mg, 2.244 mmoles), and 0.02 mM pyridoxal phosphate, in 0.1 M potassium phosphate buffer, pH 7.5, was incubated with R-transaminase AT-103 from Biocatalytics (5 mg, 44units) or cloned R-transaminase from Bacillus thuringiensis SC 16569 (0.5 mL, 10 units, BMS transaminase) at 30° C. in a total volume of 5 mL in 15 mL tubes for 44 h. Reaction yields of (R)-5,5,5-trifluoro-2-aminopentanoic acid of 49% and 48% were obtained with AT-103 and BMS transaminases, respectively. Ee was 100% in both cases.

المصدر