تفاعل #743022

معادلة التفاعل

1-(3-amino-phenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-ylamine

3-methoxypropionic acid

1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride

1-hydroxybenzotriazole

→

title compound

المردود 13.3%

N-[3-(4-Amino-pyrazolo[3,4-d]pyrimidin-1-yl)-phenyl]-3-methoxy-propionamide

المردود 13.3%

المذيبات

methanol

DMF

ظروف التفاعل

المعالجة

1. 1
   workup.STIRRINGThe reaction was stirred at room temperature for 18 hours
2. 2
   أخرىsolvent was removed in vacuo
3. 3
   غسيلThe resultant solid was washed with water
4. 4
   أخرىpurified by semi-preparative HPLC

الإجراء التجريبي

Title compound 11B, 1-(3-amino-phenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-ylamine (37 mg, 1.1 eq, 0.16 mmol) was added to a solution of 3-methoxypropionic acid (15.5 mg, 1.0 eq, 0.15 mmol), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (25 mg, 1.0 eq, 0.16 mmol), 1-hydroxybenzotriazole (20 mg, 1.0 eq, 0.15 mmol) in DMF (1 ml) which had been stirred for 10 minutes under an inert atmosphere. The reaction was stirred at room temperature for 18 hours, after which methanol (1 ml) was added, and solvent was removed in vacuo. The resultant solid was washed with water and purified by semi-preparative HPLC to yield the title compound as a white solid (5.8 mg, 0.02 mmol, 12%). LCMS: [M+H]+=313, Rt=1.35 min, 100% purity.

المصدر