تفاعل #73059

ord-0c4daffce4da4e3c88a0b888541fc707

المذيبات

ظروف التفاعل

الظروف التفصيلية
See reaction.notes.procedure_details.

المعالجة

  1. 1
    درجة الحرارةwas heated
  2. 2
    درجة الحرارةat reflux for 2 hours
  3. 3
    درجة الحرارةthe reaction mixture was heated for an additional hour
  4. 4
    أخرىThe small and larger scale reaction mixtures
  5. 5
    غسيلwashed with water (50 mL)
  6. 6
    استخلاصThe aqueous layer was extracted with dichloromethane (2×100 mL)
  7. 7
    تركيزconcentrated under reduced pressure
  8. 8
    أخرىThe residue was purified by column chromatography (silica gel eluting with a gradient of 0-10% methanol in ethyl acetate)

الإجراء التجريبي

Under a nitrogen atmosphere, tert-butyl 4 {[(3-amino-2-chloroquinolin-4-yl)amino]methyl}-4-hydroxypiperidine-1-carboxylate (0.25 g, 0.61 mmol, prepared according to the general methods of Example 4 Parts A through D using 2,4-dichloro-3-nitroquinoline in lieu of 4-chloro-3-nitroquinoline in Part C), toluene (5 mL), pyridine hydrochloride (0.02 g), and triethylorthoformate (0.11 mL, 0.65 mmol) was heated at reflux for 2 hours. More triethylorthoformate (0.5 eq) was added and the reaction mixture was heated for an additional hour. The reaction was rerun in the same manner using 5.25 g of tert-butyl 4 {[(3-amino-2-chloroquinolin-4-yl)amino]methyl}-4-hydroxypiperidine-1-carboxylate. The small and larger scale reaction mixtures were combined and washed with water (50 mL). The aqueous layer was extracted with dichloromethane (2×100 mL). The organic layers were combined and concentrated under reduced pressure. The residue was purified by column chromatography (silica gel eluting with a gradient of 0-10% methanol in ethyl acetate) to provide 4.08 g of tert-butyl 4-[(4-chloro-1H-imidazo[4,5-c]quinolin-1-yl)methyl]-4-hydroxypiperidine-1-carboxylate as a tan frothy solid.

المصدر

DOI: 10.6084/m9.figshare.5104873.v1براءة الاختراع: US08541438B2uspto-grants-2013_09