تفاعل #70809

معادلة التفاعل

methyl 6-bromo-1-isopropyl-1H-indole-4-carboxylate

1-(chloromethyl)-4-fluoro-1,4-diazoniabicyclo[2.2.2]octane ditetrafluoroborate

nitroethane

→

methyl 6-bromo-3-fluoro-1-isopropyl-1H-indole-4-carboxylate

المردود 20.7%

ظروف التفاعل

المعالجة

1. 1
   أخرىfollowed with a magnetic stir bar
2. 2
   أخرىThe vial was capped
3. 3
   أخرىReaction progress
4. 4
   أخرىAfter reaction completion the reaction solvent
5. 5
   أخرىwas removed in-vacuo
6. 6
   workup.DISSOLUTIONThe residue was dissolved with DCM (1.4 ml)
7. 7
   workup.ADDITIONThe DCM solution was charged onto an analogix Si35 SF10-8 gram column
8. 8
   غسيلThe compound was eluted with EtOAc/Hexanes, 5-15%, 20 min
9. 9
   workup.DISSOLUTIONThe mixture was dissolved with DMSO (1 ml)
10. 10
    أخرىpurified by reverse phase HPLC
11. 11
    أخرى5μ 30×100 mm column, neutral acetonitrile/0.1% formic acid in water, 50-80%, 7 min gradient
12. 12
    غسيلThe desired product eluted at 6.8 minutes
13. 13
    تركيزThe desired fractions were concentrated to a residue

الإجراء التجريبي

To a 20 ml vial was added methyl 6-bromo-1-isopropyl-1H-indole-4-carboxylate (50.0 mg, 0.169 mmol), (selectfluor) 1-(chloromethyl)-4-fluoro-1,4-diazoniabicyclo[2.2.2]octane ditetrafluoroborate (78 mg, 0.219 mmol) followed with a magnetic stir bar and nitroethane (2 ml). The vial was capped and the reaction stirred at room temperature. Reaction progress was monitored by LCMS. After reaction completion the reaction solvent was removed in-vacuo. The residue was dissolved with DCM (1.4 ml). The DCM solution was charged onto an analogix Si35 SF10-8 gram column. The compound was eluted with EtOAc/Hexanes, 5-15%, 20 min. The desired product and side MW629 co-eluted. The mixture was dissolved with DMSO (1 ml) and purified by reverse phase HPLC using a Gemini 5μ 30×100 mm column, neutral acetonitrile/0.1% formic acid in water, 50-80%, 7 min gradient. The desired product eluted at 6.8 minutes and the side product MW 629 at 9.5 minutes. The desired fractions were concentrated to a residue. LCMS and NMR support the desired structure and analytical HPLC showed 100% purity. The product, methyl 6-bromo-3-fluoro-1-isopropyl-1H-indole-4-carboxylate (11 mg, 0.035 mmol, 20.53% yield), was obtained as a white solid. 1H NMR (400 MHz, METHANOL-d4) δ 7.95 (t, J=1.77 Hz, 1H), 7.78 (d, J=1.77 Hz, 1H), 7.47 (d, J=2.53 Hz, 1H), 4.76-4.84 (m, J=1.64, 6.65, 6.65, 13.36 Hz, 1H), 3.96 (s, 3H), 1.50 (d, 6H). MS (ES)[M+H]+315.1.

المصدر