تفاعل #685032

معادلة التفاعل

FCM

triethylamine

hydrocinnamoyl chloride

→

hydrocinnamoyl-farnesyl-L-Cysteine methyl ester

Farnesyl-L-Cysteine methyl ester

المذيبات

acetone

ظروف التفاعل

المعالجة

1. 1
   أخرىThe solvent was removed under reduced pressure
2. 2
   workup.DISSOLUTIONthe residue dissolved in 150 ml of ethylacetate
3. 3
   غسيلwashed three times with water
4. 4
   تجفيفThe ethylacetate layer was dried with anhydrous sodium sulfate
5. 5
   ترشيحfiltered
6. 6
   أخرىthe ethylacetate removed under reduced pressure
7. 7
   أخرىThe residue was purified by silica gel chromatography

الإجراء التجريبي

Farnesyl-L-Cysteine methyl ester (FCM) was synthesized as described in Ma et al., Methods Enzymol. 250, 226-34 (1995). The FCM (1 g) was dissolved in approximately 100 ml of acetone followed by the addition of triethylamine (TEA, 1 ml) and 1 g of hydrocinnamoyl chloride. This was stirred for 2 h at room temperature. The solvent was removed under reduced pressure, the residue dissolved in 150 ml of ethylacetate and washed three times with water. The ethylacetate layer was dried with anhydrous sodium sulfate, filtered and the ethylacetate removed under reduced pressure. The residue was purified by silica gel chromatography to obtain hydrocinnamoyl-farnesyl-L-Cysteine methyl ester (HCFCM). The purified HCFCM was then analyzed by Electron Spray Ionization Mass Spectrometry (ESI-MS).

المصدر