تفاعل #64784

معادلة التفاعل

water

Bis(trimethylsilyl)trifluoroacetamide

L-norleucine

2-trifluoromethyl-3-acetylthiopropionyl chloride

→

N-[2-[(Acetylthio)methyl]-3,3,3-trifluoro-1-oxopropyl]-L-norleucine

المذيبات

ethyl acetate

hexane

methanol

acetonitrile

acetic acid

acetonitrile

ظروف التفاعل

المعالجة

1. 1
   workup.DISSOLUTIONalmost all of the solid dissolved
2. 2
   أخرىwas recooled to 0° C.
3. 3
   workup.STIRRINGAfter stirring at room temperature for 18 hours
4. 4
   استخلاصthe resulting mixture was extracted with ethyl acetate (500 mL)
5. 5
   workup.ADDITIONBrine was added
6. 6
   أخرىformed during this extraction
7. 7
   استخلاصThe aqueous layer was extracted once more with ethyl acetate (100 mL)
8. 8
   غسيلthe combined organic layers were washed with brine
9. 9
   تجفيفdried (MgSO4)
10. 10
    تركيزconcentrated
11. 11
    أخرىto afford an oily residue which
12. 12
    أخرىwas pre-absorbed on Celite
13. 13
    غسيلwas eluted
14. 14
    تركيزThe pure less polar fractions were concentrated
15. 15
    أخرىto afford a white solid which
16. 16
    أخرىwas recrystallized twice from ethyl acetate/hexane
17. 17
    أخرىDrying under high vacuum at room temperature for 18 hours
18. 18
    أخرىafforded 1.95 g

الإجراء التجريبي

Bis(trimethylsilyl)trifluoroacetamide (16 mL., 60 mmol.) was added to a suspension of L-norleucine (2.62 g., 20 mmol.) in 40 mL of acetonitrile at 0° C. The suspension was allowed to warm to room temperature and was stirred for 2.5 hours, at which point, almost all of the solid dissolved. The reaction mixture was recooled to 0° C. and 2-trifluoromethyl-3-acetylthiopropionyl chloride (4.7 g., 20 mmol.) was added dropwise over 20 minutes as a solution in 10 mL of acetonitrile. After stirring at room temperature for 18 hours, the reaction was poured into 200 mL of water and the resulting mixture was extracted with ethyl acetate (500 mL). Brine was added to break up an emulsion which formed during this extraction. The aqueous layer was extracted once more with ethyl acetate (100 mL) and the combined organic layers were washed with brine, dried (MgSO4), and concentrated to afford an oily residue which was pre-absorbed on Celite. The crude product and Celite mixture was loaded onto an 8×30 cm silica gel column which was eluted as follows: 5 L hexane:ethyl acetate:acetic acid (750:250:25), 2 L hexane:ethyl acetate:acetic acid (700:300:25), 2 L hexane:ethyl acetate:acetic acid (500:500:25). The pure less polar fractions were concentrated and co-evaporated from heptane to afford a white solid which was recrystallized twice from ethyl acetate/hexane. Drying under high vacuum at room temperature for 18 hours afforded 1.95 g. of the title isomer A compound as a white powder, m.p. 176°-178° C., TLC Rf =0.30 (ethyl acetate:hexane:acetic acid, 30:70:1). [α]D =-133.3° (c=0.6 methanol).

المصدر