تفاعل #64279

معادلة التفاعل

trifluoroacetic anhydride

deoxyguanosine

ammonia

→

2,6-diamino-9-(2-deoxy-D-erythro-pentofuranosyl)purine

المردود 34.0%

المذيبات

pyridine

pyridine

ظروف التفاعل

المعالجة

1. 1
   درجة الحرارةwith cooling in an ice bath
2. 2
   أخرىAfter a further 11/2 hours the mixture was evaporated to dryness
3. 3
   workup.DISSOLUTIONthe residue dissolved in water
4. 4
   ترشيحthe solution filtered through a 100 mL portion of Bio Rad AG 1-X2, hydroxide from resin
5. 5
   أخرىto remove colored impurities
6. 6
   غسيلThe resin was washed with 40% methanol in water
7. 7
   غسيلto elute crude 2,6-diamino-9-(2-deoxy-Beta-D-erythro-pentofuranosyl)purine, which
8. 8
   أخرىwas further purified on the reversed-phase column
9. 9
   أخرىEvaporation of appropriate fractions

الإجراء التجريبي

To 2 mmol of deoxyguanosine, dried by evaporation of pyridine and suspended in 20 mL of dry pyridine was added dropwise 2.3 mL (16 mmol) of trifluoroacetic anhydride, with cooling in an ice bath. After ten minutes 20 mL of cold, concentrated aqueous ammonia was added. After a further 11/2 hours the mixture was evaporated to dryness, the residue dissolved in water and the solution filtered through a 100 mL portion of Bio Rad AG 1-X2, hydroxide from resin to remove colored impurities. The resin was washed with 40% methanol in water to elute crude 2,6-diamino-9-(2-deoxy-Beta-D-erythro-pentofuranosyl)purine, which was further purified on the reversed-phase column described above using a gradient of 2-15% acetonitrile in water. Evaporation of appropriate fractions gave 192 mg (34%) of 2,6-diamino-9-(2-deoxy-D-erythro-pentofuranosyl)purine, a sample crystallized from water gave a mp of 148°. UVmax (MeOH) 282 nm, UVmin 256 nm; 1H NMR (DMSO-d6) delta (ppm) 7.91 (s, 1,H8), 6.73 (br s, 2, NH2), 6.17 ("t", 1, Japp =6 Hz, H1'), 5.73 (br s, 2, NH2), 5.25 (m, 2, 3'-OH & 5'-OH), 4.35 (m, 1, H3'), 3.84 (m, 1, H4'), 3.54 (m,2,H5',5"), 2.59 & 2.17 (m & m, 1 & 1, H2' & H2"). Anal. Calcd. for C10H14N6O3.H2O: C, 42.25; H, 5.67; N, 29,56. Found: C, 42.54; H, 5.33; N, 29.56.

المصدر