تفاعل #610980

معادلة التفاعل

ClCO2iBu

4-benzyloxy-1-dimethylcarbamoyl-isoquinoline-3-carboxylic acid

NEt3

glycine benzyl ester hydrochloride

→

title compound

[(4-Benzyloxy-1-dimethylcarbamoyl-isoquinoline-3-carbonyl)-amino]-acetic acid benzyl ester

المذيبات

CH2Cl2

ظروف التفاعل

المعالجة

1. 1
   درجة الحرارةcooled with an ice bath
2. 2
   workup.ADDITIONwas added
3. 3
   أخرىwas removed
4. 4
   workup.STIRRINGStirring
5. 5
   تركيزthe mixture was concentrated in vacuo
6. 6
   workup.ADDITIONTo the residue was added water (10 ml) and a few drops of aqueous 6N HCl
7. 7
   استخلاصThe mixture was extracted with EtOAc (15 ml)
8. 8
   تجفيفThe organic phase was dried over MgSO4
9. 9
   تركيزconcentrated in vacuo
10. 10
    أخرىto give a yellowish gum
11. 11
    أخرىPurification by flash column chromatography on silica gel

الإجراء التجريبي

To a mixture of 4-benzyloxy-1-dimethylcarbamoyl-isoquinoline-3-carboxylic acid (210 mg, 0.6 mmol), NEt3 (175 μl, 1.25 mmol), and CH2Cl2 (12 ml) cooled with an ice bath was added ClCO2iBu (83 μl, 0.63 mmol) with stirring. After stirring for 15 min glycine benzyl ester hydrochloride (127 mg, 0.63 mmol) was added and the mixture was stirred for another 15 min before the ice bath was removed. Stirring was then continued at ambient temperature for additional 1.5 h. Subsequently; the mixture was concentrated in vacuo. To the residue was added water (10 ml) and a few drops of aqueous 6N HCl. The mixture was extracted with EtOAc (15 ml). The organic phase was dried over MgSO4 and concentrated in vacuo to give a yellowish gum. Purification by flash column chromatography on silica gel using hexanes:EtOAc=7:3 as the eluent gave the title compound as a slightly yellowish gum (211 mg); MS-(+)-ion: M+1=498.1.

المصدر