تفاعل #511784

معادلة التفاعل

Boc-Val-OH

Gly-OBzl.HCl

Triethylamine

HOBt

WSC.HCl

→

Boc-Val-Gly-OBzl

المردود 90.5%

المذيبات

methylene chloride

ظروف التفاعل

المعالجة

1. 1
   تركيزThe reaction mixture was concentrated under reduced pressure
2. 2
   workup.DISSOLUTIONthe residue was dissolved in ethyl acetate (200 ml)
3. 3
   غسيلThe solution was washed with water (50 ml), 5% citric acid aqueous solution (50 ml×twice), saturated brine (50 ml), 5% sodium hydrogencarbonate aqueous solution (50 ml×twice), and saturated brine (50 ml)
4. 4
   تجفيفThe organic layer was dried over anhydrous magnesium sulfate
5. 5
   أخرىthe magnesium sulfate was removed by filtration
6. 6
   تركيزthe filtrate was concentrated under reduced pressure
7. 7
   أخرىThe residue was recrystallized from ethyl acetate/n-hexane

الإجراء التجريبي

Boc-Val-OH (8.69 g, 40.0 mmol) and Gly-OBzl.HCl (8.07 g, 40.0 mmol) were dissolved in methylene chloride (100 ml), and the solution was maintained at 0° C. Triethylamine (6.13 ml, 44.0 mmol), HOBt (1-hydroxybenzotriazole, 6.74 g, 44.0 mmol), and WSC.HCl (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, 8.44 g, 44.0 mmol) were added to the solution, and the mixture was stirred overnight at room temperature. The reaction mixture was concentrated under reduced pressure, and the residue was dissolved in ethyl acetate (200 ml). The solution was washed with water (50 ml), 5% citric acid aqueous solution (50 ml×twice), saturated brine (50 ml), 5% sodium hydrogencarbonate aqueous solution (50 ml×twice), and saturated brine (50 ml). The organic layer was dried over anhydrous magnesium sulfate, then the magnesium sulfate was removed by filtration, and the filtrate was concentrated under reduced pressure. The residue was recrystallized from ethyl acetate/n-hexane to obtain white crystals of Boc-Val-Gly-OBzl (13.2 g, 36.2 mmol).

المصدر