تفاعل #498288

ord-15f32c2071fe4df7bfa2b8a7cea54026

معادلة التفاعل

NCC(=O)O
glycine
O=[N+]([O-])c1ccc(O[C@@H]2O[C@H](CO)[C@@H](O[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@H](O)[C@H]2O)cc1
p-nitrophenyl-β-D-cellobioside
O=C1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O
gluconolactone
CC(=O)O.[Na]
sodium acetic acid
→
O=[N+]([O-])c1ccc(O)cc1
p-nitrophenol

المتفاعلات

—
solution
NCC(=O)O
glycine
O=[N+]([O-])c1ccc(O[C@@H]2O[C@H](CO)[C@@H](O[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@H](O)[C@H]2O)cc1
p-nitrophenyl-β-D-cellobioside
O=C1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O
gluconolactone
CC(=O)O.[Na]
sodium acetic acid

الكواشف

لا شيء

ظروف التفاعل

الظروف التفصيلية
See reaction.notes.procedure_details.

المعالجة

  1. 1
    أخرىan incubation of 15 minutes
  2. 2
    أخرىat 30, 40, 50, 60 or 70° C.

الإجراء التجريبي

To determine the influence of the temperature on the enzyme activity 25 μl enzyme solution was added to 225 μl of a solution containing 0.1% p-nitrophenyl-β-D-cellobioside and 25 mM gluconolactone in a 50 mM sodium acetic acid buffer at pH 5.0. Following an incubation of 15 minutes at 30, 40, 50, 60 or 70° C. the enzyme activity was stopped by adding 750 μl of a 0.5 M glycine buffer pH 9.0. The concentration of p-nitrophenol formed was determined spectrophotometrically at 400 nm.

المصدر

DOI: 10.6084/m9.figshare.5104873.v1براءة الاختراع: US06558937B1uspto-grants-2003_05