تفاعل #47358

معادلة التفاعل

N,N-diisopropylethylamine

(L)-leucine methyl ester hydrochloride

N,N-diisopropylethylamine

4-bromo-3-(2,5-difluoro-phenoxy)-but-2-enoic acid ethyl ester

→

(S)-2-[4-(2,5-difluoro-phenoxy)-2-oxo-2,5-dihydro-pyrrol-1-yl]-4-methyl-pentanoic acid methyl ester

المردود 17.4%

المذيبات

acetonitrile

acetonitrile

acetonitrile

ظروف التفاعل

المعالجة

1. 1
   workup.ADDITIONAfter addition
2. 2
   درجة الحرارةAt this time, the reaction was cooled to 25° C.
3. 3
   درجة الحرارةheated to 80° C
4. 4
   أخرىUpon reaching 80° C.
5. 5
   workup.ADDITIONAfter the addition
6. 6
   درجة الحرارةthe reaction mixture was heated to 100° C. where it
7. 7
   workup.STIRRINGstirred overnight
8. 8
   درجة الحرارةAt this time, the reaction mixture was cooled to 25° C.
9. 9
   تركيزwas concentrated in vacuo
10. 10
    workup.ADDITIONThe residue was diluted with dichloromethane (75 mL)
11. 11
    غسيلwas washed with a 2N aqueous hydrochloric acid solution (1×100 mL)
12. 12
    تجفيفa saturated aqueous sodium bicarbonate solution (1×100 mL), a saturated aqueous sodium chloride solution (1×100 mL), dried over sodium sulfate
13. 13
    ترشيحfiltered
14. 14
    تركيزconcentrated in vacuo
15. 15
    أخرىPurification by Analogix flash chromatography (40 g, 15-50% ethyl acetate/hexanes)

الإجراء التجريبي

A mixture of (L)-leucine methyl ester hydrochloride (1.28 g, 7.08 mmol) in acetonitrile (17.7 mL) was treated with N,N-diisopropylethylamine (1.15 mL, 6.95 mmol). After addition was complete, the mixture was stirred at 60° C. for 1 h. At this time, the reaction was cooled to 25° C., treated with N,N-diisopropylethylamine (1.15 mL, 6.95 mmol) and acetonitrile (17.7 mL) and then heated to 80° C. Upon reaching 80° C., the reaction was treated with a solution of 4-bromo-3-(2,5-difluoro-phenoxy)-but-2-enoic acid ethyl ester (1.56 g, 6.44 mmol) in acetonitrile (16.1 mL). After the addition was complete, the reaction mixture was heated to 100° C. where it stirred overnight. At this time, the reaction mixture was cooled to 25° C. and was concentrated in vacuo. The residue was diluted with dichloromethane (75 mL) and was washed with a 2N aqueous hydrochloric acid solution (1×100 mL), a saturated aqueous sodium bicarbonate solution (1×100 mL), a saturated aqueous sodium chloride solution (1×100 mL), dried over sodium sulfate, filtered and concentrated in vacuo. Purification by Analogix flash chromatography (40 g, 15-50% ethyl acetate/hexanes) afforded (S)-2-[4-(2,5-difluoro-phenoxy)-2-oxo-2,5-dihydro-pyrrol-1-yl]-4-methyl-pentanoic acid methyl ester (0.38 g, 17%) as a yellow oil: HR-ES-MS m/z calculated for C17H19NO4F2 [M+H]+ 340.1355, observed 340.1355; 1H NMR (300 MHz, DMSO-d6) δ ppm 0.87 (d, J=6.6 Hz, 3H), 0.92 (d, J=6.6 Hz, 3H), 1.35-1.53 (m, 1H), 1.53-1.69 (m, 1H), 1.72-1.89 (m, 1H), 3.66 (s, 3H), 4.13-4.33 (m, 2H), 4.73 (dd, J=11.5, 4.5 Hz, 1H), 5.07 (s, 1H), 7.13-7.35 (m, 1H), 7.42-7.64 (m, 2H).

المصدر