تفاعل #368129

معادلة التفاعل

3(S)-[6-amino-1(S)-ethoxycarbonylhexyl]amino-4-oxo-2,3,4,5-tetrahydro-1,5-benzoxazepine-5-acetic acid dihydrochloride

acetic acid

→

3(S)-[6-amino-1(S)-carboxyhexyl]amino-4-oxo-2,3,4,5-tetrahydro-1,5-benzoxazepine-5-acetic acid

المردود 61.5%

المذيبات

sodium hydroxide

ظروف التفاعل

المعالجة

1. 1
   غسيلeluting with methanol-water (1:10)
2. 2
   تركيزThe eluate is concentrated under reduced pressure

الإجراء التجريبي

A solution of 3(S)-[6-amino-1(S)-ethoxycarbonylhexyl]amino-4-oxo-2,3,4,5-tetrahydro-1,5-benzoxazepine-5-acetic acid dihydrochloride (0.35 g) in 1N sodium hydroxide solution (10 ml) is allowed to stand for 30 minutes at room temperature. After addition of acetic acid (2.5 ml), the mixture is subjected to Amberlite XAD-2 column chromatography eluting with methanol-water (1:10). The eluate is concentrated under reduced pressure and lyophilized to yield 3(S)-[6-amino-1(S)-carboxyhexyl]amino-4-oxo-2,3,4,5-tetrahydro-1,5-benzoxazepine-5-acetic acid (0.17 g) as colorless powder.

المصدر