تفاعل #368081

ord-c3807252a2b846b6b79e083ef66b1ba9

معادلة التفاعل

O=P([O-])(O)O.[K+]
KH2PO4
O=P([O-])([O-])O.[K+].[K+]
K2HPO4
O=C([O-])CC(O)(CC(=O)[O-])C(=O)[O-].[Na+].[Na+].[Na+]
sodium citrate
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose
NC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H](N)C[C@@H]2N)[C@H](O)[C@@H](O)[C@@H]1O
kanamycin
CC(C)C[C@H](N)C(=O)O
L-leucine
CC(C)C[C@H](N)C(=O)O
L-leucine
N=C(N)NCCC[C@H](N)C(=O)O
L-arginine
→
N[C@@H](Cc1ccccc1)C(=O)O
phenylalanine

المتفاعلات

—
(NH4)2SO4
—
MgSO4.7H2O
O=P([O-])(O)O.[K+]
KH2PO4
O=P([O-])([O-])O.[K+].[K+]
K2HPO4
O=C([O-])CC(O)(CC(=O)[O-])C(=O)[O-].[Na+].[Na+].[Na+]
sodium citrate
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose
—
L-m-fluorophenylalanine
NC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H](N)C[C@@H]2N)[C@H](O)[C@@H](O)[C@@H]1O
kanamycin
CC(C)C[C@H](N)C(=O)O
L-leucine
CC(C)C[C@H](N)C(=O)O
L-leucine
N=C(N)NCCC[C@H](N)C(=O)O
L-arginine

الكواشف

لا شيء

ظروف التفاعل

الظروف التفصيلية
See reaction.notes.procedure_details.

المعالجة

  1. 1
    أخرىprepared

الإجراء التجريبي

The cell-suspension was transferred onto a minimum medium prepared by adding 5 μg/dl kanamycin, 10 mg/dl L-leucine, 10 mg/dl L-leucine, 10 mg/dl L-arginine, 100 mg/dl D, L-m-fluorophenylalanine and 2 g/dl agar to a basal minimum medium of pH 7.2 containing 0.6 g/dl KH2PO4, 1.4 g/dl K2HPO4, 0.2 g/dl (NH4)2SO4, 0.1 g/dl sodium citrate, 0.02 g/dl MgSO4.7H2O, and 0.5 g/dl glucose. After 3 days cultivation at 37° C., two colonies appeared on the agar-medium.

المصدر

DOI: 10.6084/m9.figshare.5104873.v1براءة الاختراع: US04591562uspto-grants-1986_05