تفاعل #367969

معادلة التفاعل

Dibenzylphosphoryl-L-alanyl-N-(2-indanyl)-glycyl-L-proline

L-arginine

→

dibenzylphosphoryl-L-alanyl-N-(2-indanyl)glycyl-L-proline L-arginine salt

المردود 85.2%

المذيبات

ethanol

water

ظروف التفاعل

المعالجة

1. 1
   أخرىthe aqueous solution produced
2. 2
   workup.ADDITIONwas added
3. 3
   workup.DISTILLATIONThe solvent was distilled off under reduced pressure
4. 4
   workup.ADDITIONwater was added
5. 5
   تجفيفThis water solution was freeze-dried

الإجراء التجريبي

Dibenzylphosphoryl-L-alanyl-N-(2-indanyl)-glycyl-L-proline (0.77 g, 1.2 m mole) was dissolved in ethanol (4 ml), and the aqueous solution produced by dissolving L-arginine (0.25 g, 1.2 m mole) in water (4 ml), was added thereto. The solvent was distilled off under reduced pressure, and then water was added thereto. This water solution was freeze-dried to obtain dibenzylphosphoryl-L-alanyl-N-(2-indanyl)glycyl-L-proline L-arginine salt (0.84 g, yield: 85.2%). This product gave a single spot having an Rf =0.2 for the peptide in free form, and an Rf =0 for arginine, respectively, by thin layer chromatography (developing solvent, acetonitrile:acetic acid=30:1, Colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrine, and heating).

المصدر