تفاعل #354108

معادلة التفاعل

1-[2-(4-nitrophenyl)ethyl]-1,4,7,10-tetraazacyclododecane-4,7,10-triacetic acid, triethyl ester

NaOH

→

1-[2-(4-nitrophenyl)ethyl]-1,4,7,10-tetraazacyclododecane-4,7,10-triactic acid

المذيبات

water

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المعالجة

1. 1
   درجة الحرارةThe resulting homogeneous orange solution was then cooled to room temperature
2. 2
   أخرىfreeze-dried
3. 3
   أخرىto yield a brown solid which
4. 4
   أخرىwas purified by HPLC (Q-Sepharose™, HPLC System III)
5. 5
   أخرىover 60 minutes

الإجراء التجريبي

To a suspension of 200 mg (0.35 mmole) of 1-[2-(4-nitrophenyl)ethyl]-1,4,7,10-tetraazacyclododecane-4,7,10-triacetic acid, triethyl ester (prepared by the procedure of Example 19) in 15 ml of water was added 59 μl of NaOH (50% wt/wt, 3 eq). The mixture was stirred at 80° C. for 6 hours. The resulting homogeneous orange solution was then cooled to room temperature and freeze-dried to yield a brown solid which was purified by HPLC (Q-Sepharose™, HPLC System III) using a linear gradient of 0-10% aqueous HOAc over 60 minutes to yield (50%) of the hydrolyzed product 1-[2-(4-nitrophenyl)ethyl]-1,4,7,10-tetraazacyclododecane-4,7,10-triactic acid, and characterized by:

المصدر