تفاعل #336577

ord-bffb7174a0ac4af9a4dc20f013edb573

معادلة التفاعل

O=C[C@H](O)[C@H](O)[C@H](O)CO
D-ribose
O=CC(O)C(O)C(O)CO
pentose
NCCCC[C@H](N)C(=O)O
L-lysine
N=C(N)NCCC[C@H](N)C(=O)O
L-arginine
→
N[C@@H](CCCCn1cccc2nc(NCCC[C@H](N)C(=O)O)nc1-2)C(=O)O
Pentosidine

المتفاعلات

O=C[C@H](O)[C@H](O)[C@H](O)CO
D-ribose
O=CC(O)C(O)C(O)CO
pentose
NCCCC[C@H](N)C(=O)O
L-lysine
N=C(N)NCCC[C@H](N)C(=O)O
L-arginine

الكواشف

لا شيء

ظروف التفاعل

درجة الحرارة
80°CELSIUS
الظروف التفصيلية
See reaction.notes.procedure_details.

المعالجة

  1. 1
    أخرىthe synthetic fluorophore was prepared preparatively
  2. 2
    أخرىpurified

الإجراء التجريبي

In order to confirm the pentose-derived nature of the native fluorophore 100 mM each of L-lysine, L-arginine and D-ribose were heated for 1 hour at 80° C. Injection of a small amount of this synthetic material on HPLC revealed a major fluorescent peak co-eluting with the native fluorophore. (see FIG. 5) In order to substantiate the proposed structure, the synthetic fluorophore was prepared preparatively and purified. Total yield was 21 mg; i.e., 0.02% of the reactants.

المصدر

DOI: 10.6084/m9.figshare.5104873.v1براءة الاختراع: US05214138uspto-grants-1993_05