تفاعل #328710

ord-75fe930b28d34ffb8011e18951ae6050

معادلة التفاعل

N=C(N)NCCC[C@H](N)C(=O)O
L-arginine
N=C(N)NCCC[C@H](N)C(=O)O
L-arginine
N=C(N)NCCC[C@H](N)C(=O)O
L-arginine
CCCCc1nc(Cl)c(CO)n1Cc1ccc(-c2ccccc2-c2nn[nH]n2)cc1
Losartan
CC[C@H](C)[C@H](NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@@H](NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@@H](N)CC(=O)O)C(C)C)C(=O)N[C@@H](Cc1cnc[nH]1)C(=O)N1CCC[C@H]1C(=O)O
Angiotensin-(1-7)
CC[C@H](C)[C@H](NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@@H](NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@@H](N)CC(=O)O)C(C)C)C(=O)N[C@@H](Cc1cnc[nH]1)C(=O)N1CCC[C@H]1C(=O)O
Ang-(1-7)
→
CCCCc1nc(Cl)c(CO)n1Cc1ccc(-c2ccccc2-c2nn[nH]n2)cc1.CC[C@H](C)[C@H](NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@@H](NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@@H](N)CC(=O)O)C(C)C)C(=O)N[C@@H](Cc1cnc[nH]1)C(=O)N1CCC[C@H]1C(=O)O.N=C(N)NCCC[C@H](N)C(=O)O
Losartan Ang-(1-7) Arginine

المتفاعلات

N=C(N)NCCC[C@H](N)C(=O)O
L-arginine
N=C(N)NCCC[C@H](N)C(=O)O
L-arginine
N=C(N)NCCC[C@H](N)C(=O)O
L-arginine
CCCCc1nc(Cl)c(CO)n1Cc1ccc(-c2ccccc2-c2nn[nH]n2)cc1
Losartan
CC[C@H](C)[C@H](NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@@H](NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@@H](N)CC(=O)O)C(C)C)C(=O)N[C@@H](Cc1cnc[nH]1)C(=O)N1CCC[C@H]1C(=O)O
Angiotensin-(1-7)
CC[C@H](C)[C@H](NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@@H](NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@@H](N)CC(=O)O)C(C)C)C(=O)N[C@@H](Cc1cnc[nH]1)C(=O)N1CCC[C@H]1C(=O)O
Ang-(1-7)
—
final solution

الكواشف

لا شيء

المذيبات

O
water
O
water

ظروف التفاعل

الظروف التفصيلية
See reaction.notes.procedure_details.

المعالجة

  1. 1
    workup.ADDITIONwere added
  2. 2
    درجة الحرارةfrozen in liquid nitrogen

الإجراء التجريبي

The Ang-(1-7) losartanate compound (PID) was weighed and placed in a beaker to which a few milliliters of Milli-Q water were added. Since this compound is insoluble in water a turbid solution was observed having suspended particles. L-arginine was added to the solution under agitation, at a molar ratio of 4:1:1 (L-arginine:Losartan:Angiotensin-(1-7)), and a total clearing of the solution was observed, which indicated the solubility of the compound after the interaction with L-arginine. The final solution was placed in eppendorf tubes and frozen in liquid nitrogen, being submitted to the lyophilization process for 24 hours. The compound was called PAD and was submitted to physicochemical characterization.

المصدر

DOI: 10.6084/m9.figshare.5104873.v1براءة الاختراع: US08653031B2uspto-grants-2014_02