تفاعل #305372

معادلة التفاعل

dimethylamine

triethylamine

1-[3-(4-tert-butyl-benzenesulfonylamino)-5-chloro-pyridin-2-yl]-1H-pyrazole-4-carboxylic acid ethyl ester

NaOH

→

1-[3-(4-tert-butyl-benzenesulfonylamino)-5-chloro-pyridin-2-yl]-1H-pyrazole-4-carboxylic acid dimethylamide

المذيبات

acetic acid

THF

ظروف التفاعل

المعالجة

1. 1
   أخرىThe vial was sealed
2. 2
   استخلاصThe mixture was extracted with ethyl acetate (2×10 mL)
3. 3
   تركيزthe combined organics were concentrated in vacuo
4. 4
   workup.DISSOLUTIONThe crude product was subsequently dissolved in 2 mL of THF in a 25 mL scintillation vial
5. 5
   أخرىThe vial was sealed
6. 6
   workup.STIRRINGstirred at ambient temperature for 1 hour
7. 7
   أخرىThe volatiles were then evacuated in vacuo
8. 8
   أخرىthe residue was purified via preparative HPLC

الإجراء التجريبي

A 25 mL scintillation vial was charged with 1-[3-(4-tert-butyl-benzenesulfonylamino)-5-chloro-pyridin-2-yl]-1H-pyrazole-4-carboxylic acid ethyl ester (synthesized according to general procedure D, 93 mg, 0.2 mmol), NaOH (2 mL, 1.0 M solution in water), and THF (3 mL). The vial was sealed and stirred at 80° C. for 16 hours. The reaction solution was subsequently neutralized to pH=5 with glacial acetic acid. The mixture was extracted with ethyl acetate (2×10 mL) and the combined organics were concentrated in vacuo. The crude product was subsequently dissolved in 2 mL of THF in a 25 mL scintillation vial. To the resultant solution was added with dimethylamine (0.2 mL, 2.0 M in THF), 1-propanephosphonic anhydride solution (184 mg, 50% solution in ethyl acetate), and triethylamine (41 mg, 0.4 mmol). The vial was sealed and stirred at ambient temperature for 1 hour. The volatiles were then evacuated in vacuo and the residue was purified via preparative HPLC to afford 1-[3-(4-tert-butyl-benzenesulfonylamino)-5-chloro-pyridin-2-yl]-1H-pyrazole-4-carboxylic acid dimethylamide as a white powder: MS (ES) M+H expected 462.1, found 462.0.

المصدر