تفاعل #2605

معادلة التفاعل

sodium bicarbonate

Boc-L-alanine

4-Aminomethyl-N-{2-[4-(4-fluorobenzoyl)piperidino]ethyl}-N-(2-methoxyethyl)benzamide

EDC hydrochloride

→

title compound

المردود 72.6%

4-[(S)-2-(tert-Butoxycarbonylamino)propionyl]aminomethyl-N-{2-[4-(4-fluorobenzoyl)piperidino]ethyl}-N-(2-methoxyethyl)benzamide

المردود 72.6%

المذيبات

water

methylene chloride

ظروف التفاعل

المعالجة

1. 1
   درجة الحرارةwhile cooling in an ice bath
2. 2
   استخلاصextracted with methylene chloride
3. 3
   تجفيفThereafter, the resulting organic layer was dried on anhydrous sodium carbonate
4. 4
   أخرىthe solvent was removed by evaporation
5. 5
   أخرىthe resulting residue was purified by a silica gel column chromatography (ethyl acetate)

الإجراء التجريبي

4-Aminomethyl-N-{2-[4-(4-fluorobenzoyl)piperidino]ethyl}-N-(2-methoxyethyl)benzamide (120 mg, 0.245 mmol) was dissolved in methylene chloride (5 ml) to which were subsequently added Boc-L-alanine (46.4 mg, 0.245 mmol) and EDC hydrochloride (52 mg, 0.270 mmol) while cooling in an ice bath. After 4 hours of stirring at the same temperature, the reaction solution was mixed with water (10 ml), adjusted to pH 8 with saturated sodium bicarbonate aqueous solution and then extracted with methylene chloride. Thereafter, the resulting organic layer was dried on anhydrous sodium carbonate, the solvent was removed by evaporation, and the resulting residue was purified by a silica gel column chromatography (ethyl acetate) to obtain 109 mg (68.9%) of the title compound in a colorless amorphous form.

المصدر