تفاعل #2336539

معادلة التفاعل

D-5-benzylhydantoin

H3PO4

→

N-carbamylphenylalanine

ظروف التفاعل

المعالجة

1. 1
   workup.WAITcentrifuging at 20,000 G for 10 minutes
2. 2
   أخرىto remove the pellet

الإجراء التجريبي

The assay of the D-hydantoinase activity was conducted by incubating a reaction mixture containing 120 mg/dl D-5-benzylhydantoin (BH), 50 mM KPB (pH 8.0) and an enzyme solution at 37° C. for 30 minutes, adding 9 volumes of 1.1 mM CuSO4, 11.1 mM H3PO4, centrifuging at 20,000 G for 10 minutes to remove the pellet, and then quantifying the resultant N-carbamylphenylalanine (N-Car-Phe) by HPLC. One unit of the enzyme activity was defined as an enzymatic activity capable of producing 1 μmol of N-carbamylphenylalanine per 1 minute under the condition described above.

المصدر