تفاعل #2200578
ord-22dfff47d7d344e4866063dcc9b46fe2
معادلة التفاعل
sodium benzoate
citric acid
vanillic acid
→
guaiacol
الكواشف
لا شيء
ظروف التفاعل
الظروف التفصيلية
See reaction.notes.procedure_details.
المعالجة
- 1workup.ADDITIONadded
- 2workup.ADDITIONtwo steps of preparations containing 103 and 105 spores/ml of A
- 3أخرىacidoterrestris spores were prepared
- 4أخرىcultured at 45° C.
- 5أخرىAs a control, a sample prepared
- 6workup.ALIQUOTThe resulting solution was sampled with a lapse of time
الإجراء التجريبي
Using A. acidoterrestris-proliferative acid sport drink (comprising 1% fruit juice, 0.18% citric acid, 9% isomerized sugar and 0.25% flavor) containing 100 ppm of vanillic acid added therein, two steps of preparations containing 103 and 105 spores/ml of A. acidoterrestris spores were prepared therein and cultured at 45° C. with shaking. As a control, a sample prepared by adding 300 ppm of sodium benzoate having the effect of inhibiting the growth of the same bacteria to the same solution was used. The resulting solution was sampled with a lapse of time and the amount of guaiacol produced was measured by the Peroxidase method.