تفاعل #217754

معادلة التفاعل

2,6-Diamino-9-(2-fluoro-β-D-arabinosyl)purine

adenosine

→

title compound

المردود 100.0%

9-(2-Fluoro-β-D-arabinosyl)guanine

المردود 100.0%

المذيبات

Tris-HCl

ظروف التفاعل

المعالجة

1. 1
   أخرىThe resultant reaction mixture
2. 2
   ترشيحwas subjected to filtration by use of a membrane
3. 3
   ترشيحfilter (PTFE, 0.5 μm)
4. 4
   أخرىthe filtrate was prepared into 60 mL of an aqueous solution
5. 5
   أخرىThe aqueous solution was purified by means of reverse-phase ODS column chromatography (80 mL, 0 to 2% aqueous acetonitrile)

الإجراء التجريبي

2,6-Diamino-9-(2-fluoro-β-D-arabinosyl)purine (61.2 mg, 0.21 mmol) was dissolved in 50 mM Tris-HCl buffer solution (20 mL, pH 7.0), and adenosine deaminase (71 units) was added to the resultant solution, followed by stirring at room temperature for 1.5 hours. The resultant reaction mixture was subjected to filtration by use of a membrane filter (PTFE, 0.5 μm), and the filtrate was prepared into 60 mL of an aqueous solution. The aqueous solution was purified by means of reverse-phase ODS column chromatography (80 mL, 0 to 2% aqueous acetonitrile), to thereby yield 60.4 mg of the title compound as colorless crystals (yield: 100%).

المصدر