تفاعل #216081
ord-6192c1e9fdbe40cba892f641eff308c6
الكواشف
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المذيبات
ظروف التفاعل
الظروف التفصيلية
See reaction.notes.procedure_details.
الإجراء التجريبي
To make the succinyl CS-045 conjugate of β-phycoerythrin, first 44 mg of CS-045 was reacted for 5 hours at room temperature with 20 mg succinic anhydride in pyridine. Then 4 mg of the resulting succinyl CS-045 was reacted overnight at 4° C. with 2 mg EDC and 1.2 mg N-hydroxysuccinimide to produce CS-045 succinimide. Forty micrograms of this CS-045 succinimide was reacted for 4 hours at room temperature with 500 μg β-phycoerythrin. The final succinyl CS-045 conjugate of β-phycoerythrin was precipitated with 60% final (w/v) saturated ammonium sulfate and resuspended in phosphate buffered saline.