تفاعل #2006451

معادلة التفاعل

sodium hydroxide

N-Boc-3-pyrrolidinone

D-glucose

D-alanine

pyridoxal phosphate

N-Boc-3-pyrrolidinone

D-glucose

NAD+

D-alanine

pyridoxal phosphate

→

(R)—N-Boc-3-aminopyrrolidine

ظروف التفاعل

المعالجة

1. 1
   أخرى(pNTASPAG), obtained in Example 11, which
2. 2
   workup.ADDITIONwas added so that total volume was 25 ml
3. 3
   workup.ADDITIONwere added

الإجراء التجريبي

To a flask containing 0.5 g of a substrate N-Boc-3-pyrrolidinone, 0.73 g of D-glucose, 4 mg of NAD+, 1.44 g of D-alanine, and 3.3 mg of pyridoxal phosphate, a culture fluid of the recombinant E. coli HB101 (pNTASPAG), obtained in Example 11, which expresses TAS, PALDH, and GDH was added so that total volume was 25 ml. The resultant product was stirred at 30° C. while being adjusted to a pH of 6.8 by drippage of 5 N aqueous solution of sodium hydroxide. After nine hours in reaction, 0.25 g of N-Boc-3-pyrrolidinone, 0.37 g of D-glucose, 0.37 g of D-alanine, and 3 mg of pyridoxal phosphate were added.

المصدر