تفاعل #1951041

معادلة التفاعل

ammonia

Compound 7

3′,5′-di-O-acetyl-4′-C-cyano-2′-deoxy-2-fluoroadenosine

→

4′-C-cyano-2′-deoxy-2-fluoroadenosine

المذيبات

methanol

ظروف التفاعل

المعالجة

1. 1
   أخرىThe resultant reaction mixture
2. 2
   تركيزwas concentrated under reduced pressure
3. 3
   أخرىthe resultant residue was purified by means of silica gel column chromatography (silica gel 10 ml, hexane/ethyl acetate (5:1), ethyl acetate, ethyl acetate/methanol (10:1))

الإجراء التجريبي

Compound 7 (53.7 mg, 0.142 mmol) was dissolved in methanol (1.90 ml), and 28% aqueous ammonia (1.30 ml) was added to the resultant solution, followed by stirring at room temperature for 30 minutes. The resultant reaction mixture was concentrated under reduced pressure, and then the resultant residue was purified by means of silica gel column chromatography (silica gel 10 ml, hexane/ethyl acetate (5:1), ethyl acetate, ethyl acetate/methanol (10:1)), to thereby yield compound 8 (30.2 mg, 0.10 mmol, 72.3%).

المصدر