تفاعل #1755459
ord-01cba8bec4c749768425947ff25e45f9
معادلة التفاعل
Tris HCl
NADP
magnesium chloride
6-phosphogluconic acid
→
NADPH
الكواشف
لا شيء
ظروف التفاعل
الظروف التفصيلية
See reaction.notes.procedure_details.
المعالجة
- 1أخرىThe resulting mixture was subjected to reaction in a 1-ml cuvette at 30° C.
- 2درجة الحرارةthe increase in the absorbance
الإجراء التجريبي
The crude enzyme solution was added to a reaction mixture comprising 50 mmol/l Tris/HCl (pH 7.5), 0.5 mmol/l NADP, 10 mmol/l magnesium chloride and 2 mmol/l 6-phosphogluconic acid to make up to 1.5 ml. The resulting mixture was subjected to reaction in a 1-ml cuvette at 30° C. and the increase in the absorbance was measured at 340 nm. On the basis of the molar extinction coefficient of NADPH (6220), the amount of NADPH formed per minute was calculated. GND specific activity was expressed in terms of the amount of NADPH formed per mg of protein per minute.