تفاعل #1702031

معادلة التفاعل

topotecan hydrochloride

sodium

cysteic acid

→

topotecan

المذيبات

Methanol

ظروف التفاعل

المعالجة

1. 1
   أخرىevaporated in vacuo
2. 2
   workup.ADDITION120 ml sodium chloride solution (9 mg/ml) was added to the residue
3. 3
   أخرىobtained
4. 4
   أخرىafter evaporation
5. 5
   أخرىtransparent (approx. 20 min)
6. 6
   أخرىThe solution obtained
7. 7
   ترشيحwas filtered through 0.2 mm
8. 8
   ترشيحfilter
9. 9
   ترشيحThe filtration

الإجراء التجريبي

Methanol stock-solutions of topotecan hydrochloride (120 ml, 1.09 mg/ml) and sodium salt of methyl ester of N-all-trans-retinoyl cysteic acid (32 ml, 15 mg/ml) were mixed in a 500 ml round-bottom flask and evaporated in vacuo. 120 ml sodium chloride solution (9 mg/ml) was added to the residue obtained after evaporation, and the mixture was stirred until it became clear and transparent (approx. 20 min). The concentration of topotecan in the obtained solution was 1 mg/ml, corresponding to a topotecan hydrochloride concentration of 1.09 mg/ml. The solution obtained was filtered through 0.2 mm filter. The filtration did not result in reduction of topotecan concentration.

المصدر