تفاعل #166299
ord-a2ff19afe194443399156c5428e7ed28
معادلة التفاعل
المتفاعلات
المذيبات
ظروف التفاعل
المعالجة
- 1أخرىThis mixture was degassed
- 2أخرىby bubbling nitrogen through this solution for ˜2 min
- 3درجة الحرارةwas heated overnight at 100° C
- 4درجة الحرارةThe reaction was cooled to 20° C.
- 5أخرىThe layers were partitioned
- 6غسيلthe aqueous layer was washed with ethyl acetate
- 7تجفيفThe combined organic fractions were dried over MgSO4
- 8تركيزconcentrated
- 9أخرىThe product was purified by flash column chromatography
- 10غسيلeluting with a gradient of DCM-6% methanol/0.3% NH4OH/DCM
الإجراء التجريبي
Compound 4 (0.075 g, 0.30 mmol) was combined with sodium 2-methylpropan-2-olate (0.085 g, 0.89 mmol) and compound 72 (0.14 g, 0.38 mmol) in toluene (2 mL). This mixture was degassed by bubbling nitrogen through this solution for ˜2 min followed by the addition of Pd2(dba)3 (0.014 g, 0.015 mmol) and t-Butyl-XPhos (0.025 g, 0.059 mmol). The reaction was heated overnight at 60° C. Additional compound 72, 0.28 g, 0.76 mmol), Pd2(dba)3 (25 mg), t-Butyl-XPhos (54 mg), and the sodium 2-methylpropan-2-olate (0.085 g, 0.89 mmol) were added to the reaction which was heated overnight at 100° C. The reaction was cooled to 20° C. and poured into a separation funnel, with ethyl acetate and water. The layers were partitioned and the aqueous layer was washed with ethyl acetate. The combined organic fractions were dried over MgSO4 and concentrated. The product was purified by flash column chromatography, eluting with a gradient of DCM-6% methanol/0.3% NH4OH/DCM to yield compound 74 (95 mg) as a brown film. LCMS-ESI (POS), M/Z, M+1: Found 549.3.