تفاعل #11966

معادلة التفاعل

Nα-Fmoc-Nδ-Boc-L-ornithine

WSCI hydrochloride

HOBt

→

title compound

المردود 102.8%

Nα-Fmoc-Nδ-Boc-L-ornithine methyl ester

المردود 102.8%

المذيبات

methanol

methanol

ظروف التفاعل

المعالجة

1. 1
   أخرىwas removed
2. 2
   استخلاصthe residue was extracted with chloroform
3. 3
   غسيلThe extract was washed with water, saturated aqueous solution of sodium hydrogencarbonate, and saturated brine
4. 4
   تجفيفdried over anhydrous sodium sulfate
5. 5
   أخرىto remove water
6. 6
   أخرىSyrup obtained
7. 7
   أخرىby removing the solvent
8. 8
   أخرىwas purified by silica gel column chromatography (50 g, chloroform/ethyl acetate=5/1)

الإجراء التجريبي

Commercially available Nα-Fmoc-Nδ-Boc-L-ornithine (1.00 g), WSCI hydrochloride (0.633 g), and HOBt (0.446 g) were reacted in methanol (20 ml) for 23 hours at room temperature. After the reaction, methanol was removed and the residue was extracted with chloroform. The extract was washed with water, saturated aqueous solution of sodium hydrogencarbonate, and saturated brine, and dried over anhydrous sodium sulfate to remove water. Syrup obtained by removing the solvent was purified by silica gel column chromatography (50 g, chloroform/ethyl acetate=5/1) to obtain the title compound (1.06 g) as a white solid.

المصدر