تفاعل #1114534

ord-afa5e19ef9954e55a90885585ba46ecf

معادلة التفاعل

O=C(N[C@H](CO)[C@H](O)c1ccc([N+](=O)[O-])cc1)C(Cl)Cl
chloramphenicol
N[C@@H](Cc1ccc(O)cc1)C(=O)O
L-tyrosine
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose
NC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H](N)C[C@@H]2N)[C@H](O)[C@@H](O)[C@@H]1O
kanamycin
[Cl-].[NH4+]
NH4Cl
→
N[C@@H](Cc1ccccc1)C(=O)O
L-phenylalanine

المتفاعلات

O=C(N[C@H](CO)[C@H](O)c1ccc([N+](=O)[O-])cc1)C(Cl)Cl
chloramphenicol
N[C@@H](Cc1ccc(O)cc1)C(=O)O
L-tyrosine
O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
glucose
NC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H](N)C[C@@H]2N)[C@H](O)[C@@H](O)[C@@H]1O
kanamycin
[Cl-].[NH4+]
NH4Cl

الكواشف

لا شيء

المذيبات

O=S(=O)(O)CCCN1CCOCC1
MOPS

ظروف التفاعل

الظروف التفصيلية
See reaction.notes.procedure_details.

المعالجة

  1. 1
    أخرىwere performed at 37° C. with 225 rpm
  2. 2
    workup.ADDITIONWhen appropriate, antibiotics were added in the following concentrations

الإجراء التجريبي

L-tyrosine production experiments were performed at 37° C. with 225 rpm orbital shaking in 50 ml MOPS minimal medium (Teknova) (Neidhardt, Bloch et al. 1974) cultures supplemented with 5 g/l glucose and an additional 4 g/l NH4Cl. All liquid cultivations were conducted in at least triplicates. When appropriate, antibiotics were added in the following concentrations: 34 μg/ml chloramphenicol for maintenance of pHACM-derived plasmids and 20 μg/ml kanamycin for maintenance of pZE-derived plasmids. L-phenylalanine auxotrophic (ΔpheA) cultures were additionally provided with L-phenylalanine (Sigma) at a concentration of 0.35 mM.

المصدر

DOI: 10.6084/m9.figshare.5104873.v1براءة الاختراع: US08735132B2uspto-grants-2014_05